1H3G
Cyclomaltodextrinase from Flavobacterium sp. No. 92: from DNA sequence to protein structure
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-03 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 181.339, 181.339, 231.511 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.000 * - 2.100 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.22300 |
Structure solution method | MAD |
RMSD bond length | 0.016 * |
RMSD bond angle | 1.340 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | 2.180 |
High resolution limit [Å] | 2.100 | 2.080 * |
Rmerge | 0.060 | 0.400 * |
Total number of observations | 629678 * | |
Number of reflections | 86572 | 8625 * |
<I/σ(I)> | 10.4 | 1.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
3 | 1 | reservoir | 3.8 (M) |