1GXY
crystal structure of the eucaryotic mono-ADP-ribosyltransferase ART2.2; CRYSTAL FORM A (P21)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.510, 85.640, 57.960 |
Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
Resolution | 35.000 * - 1.700* |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.22300 * |
Structure solution method | SIRAS |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.380 * |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 1.800 |
High resolution limit [Å] | 1.700 * | 1.710 |
Rmerge | 0.034 * | 0.119 * |
Total number of observations | 195638 * | |
Number of reflections | 48705 | 6787 * |
<I/σ(I)> | 10.5 | 6 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.3 | 293 * | Mueller-Dieckmann, C., (2002) Acta Crystallogr., D58, 1211. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.8 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.3 |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | PEG4000 | 22 (%(w/v)) |