1GWW
ALPHA-,1,3 GALACTOSYLTRANSFERASE - ALPHA-D-GLUCOSE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.163, 94.370, 94.760 |
| Unit cell angles | 90.00, 99.40, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.22300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4v |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.463 |
| Total number of observations | 236674 * | |
| Number of reflections | 65613 | |
| <I/σ(I)> | 21 | 3.5 |
| Completeness [%] | 90.5 | 84.8 |
| Redundancy | 10 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 16 * | PEG6000, TRIS/HCL, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | MES/NaOH | 20 (mM) | pH6.0 |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | UDP | 10 (mM) | |
| 5 | 1 | drop | 0.1 (mM) | ||
| 6 | 1 | reservoir | PEG6000 | 5 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
