1GSH
STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Detector technology | IMAGE PLATE |
Collection date | 1991-03-18 |
Detector | RIGAKU |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 87.800, 87.800, 170.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 23.50000 * |
RMSD bond length | 0.012 |
RMSD bond angle | 25.359 * |
Data reduction software | WEIS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.070 |
Number of reflections | 30967 |
Completeness [%] | 84.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 | CRYSTALS WERE PREPARED AT PH 7.5 BY MICRODIALYSIS METHOD WITH AMMONIUM SULFATE AS THE PRECIPITATING AGENT. THE INNER SOLUTION 100 MICROLITER CONTAINED 1.5 % (W/V) GSHASE, 5 MM MGCL2 AND 10 % SATURATED AMMONIUM SULFATE IN 50 MM TRIS-HCL BUFFER (PH 7.5). THE INNER SOLUTION WAS DIALYZED AGAINST 50 MM TRIS-HCL BUFFER (PH 7.5) WHICH CONTAINED 5 MM MGCL2 AND 25 % SATURATED AMMONIUM SULFATE.TRIS-HCL BUFFER, microdialysis |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Tris-HCl | 50 (mM) | |
2 | 1 | 1 | 5 (mM) | ||
3 | 1 | 1 | ammonium sulfate | 25 (%sat) |