1GN4
H145E mutant of Mycobacterium tuberculosis iron-superoxide dismutase.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.660, 102.860, 73.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
Rwork | 0.172 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ids |
RMSD bond length | 0.012 |
RMSD bond angle | 0.025 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | CCP4 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | |
High resolution limit [Å] | 2.500 | |
Rmerge | 0.107 | 0.297 |
Number of reflections | 207000 | |
Completeness [%] | 94.4 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | 100MM TRIS-HCL PH 7.0, 25% PEG 6000,PROTEIN CONCENTRATION = 3 MG/ML, 4 DEGREES CENTIGRADE. |