1GHR

THE THREE-DIMENSIONAL STRUCTURES OF TWO PLANT BETA-GLUCAN ENDOHYDROLASES WITH DISTINCT SUBSTRATE SPECIFICITIES

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	87.400, 87.400, 109.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.176
Rwork	0.176
RMSD bond length	0.012
RMSD bond angle	1.620
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.101 *
Total number of observations	86286 *
Number of reflections	16399 *
Completeness [%]	82.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop	ammonium sulfate	25 (%sat)
4	1	reservoir	ammonium sulfate	45 (%sat)