1GGI
CRYSTAL STRUCTURE OF AN HIV-1 NEUTRALIZING ANTIBODY 50.1 IN COMPLEX WITH ITS V3 LOOP PEPTIDE ANTIGEN
Experimental procedure
Spacegroup name | P 1 21 1 |
Unit cell lengths | 130.330, 52.570, 82.040 |
Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
Resolution | 10.000 * - 2.800 |
R-factor | 0.188 |
Rwork | 0.188 |
RMSD bond length | 0.018 |
RMSD bond angle | 3.790 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.100 * |
Completeness [%] | 81.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Fab | 7.5 (mg/ml) | |
2 | 1 | drop | MP1 peptide | 2.5 (mg/ml) | |
3 | 1 | reservoir | imidazole malate | 0.2 (M) | |
4 | 1 | reservoir | PEG10000 | 14 (%) |