1GDN
FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-03-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.156, 36.763, 39.616 |
| Unit cell angles | 102.19, 104.70, 102.91 |
Refinement procedure
| Resolution | 20.000 - 0.810 |
| R-factor | 0.1083 |
| Rwork | 0.108 |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 0.036 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.820 |
| High resolution limit [Å] | 0.810 | 0.810 |
| Rmerge | 0.030 | 0.267 |
| Total number of observations | 700099 * | |
| Number of reflections | 156398 * | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 90.9 | 81.9 |
| Redundancy | 4.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | sodium sulphate, sodium citrate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (M) | |
| 2 | 1 | drop | 3,3-dimethyl glutaric acid | 10 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | reservoir | 1.4 (M) | ||
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 7 | 1 | reservoir | 2-propanol | 0-5 (%) |
