1G82
STRUCTURE OF FIBROBLAST GROWTH FACTOR 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-12-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.07 |
| Spacegroup name | I 41 |
| Unit cell lengths | 151.947, 151.947, 117.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.2103 |
| Rwork | 0.208 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2afg |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.057 | 0.242 |
| Number of reflections | 40985 | |
| <I/σ(I)> | 8.5 | 3.3 |
| Completeness [%] | 99.9 | 99.9 * |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 292 | Ammonium sulfate, MES, TRIS, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.1 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2.0 (M) | |
| 3 | 1 | drop | MES/Tris | 0.1 (M) |
