1G7R
X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2000-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.316, 54.355, 90.978 |
| Unit cell angles | 104.90, 101.33, 98.75 |
Refinement procedure
| Resolution | 22.000 - 2.200 |
| Rwork | 0.243 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.023 | 0.150 |
| Number of reflections | 79984 | |
| <I/σ(I)> | 30 | |
| Completeness [%] | 92.0 | 92 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 0.1M Cacodylate 5.8, 18% PEG4000, 0.2M Lithium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 0.2 (M) | ||
| 3 | 1 | reservoir | cacodylate | 100 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 18 (%) |
