1G1M
ALL-FERROUS NITROGENASE IRON PROTEIN FROM AZOTOBACTER VINELANDII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 113.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.000, 93.300, 62.800 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.250 |
| Rwork | 0.238 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.390 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.054 | 0.306 * |
| Total number of observations | 154673 * | |
| Number of reflections | 28724 | |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 93.2 | 85.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 295 | 26-29% PEG 4000, 700-900 mM NaCl, 100 mM Tris (pH 8.0), 20% glycerol, capillary microbatch method at 295 K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 26-29 (%) | |
| 2 | 1 | 1 | 700-900 (mM) | ||
| 3 | 1 | 1 | Tris | 100 (mM) | |
| 4 | 1 | 2 | protein | 30 (mg/ml) | |
| 5 | 1 | 2 | glycerol | 20 (%) | |
| 6 | 1 | 2 | 450 (mM) | ||
| 7 | 1 | 2 | Tris | 50 (mM) |
