1FZK
MHC CLASS I NATURAL MUTANT H-2KBM1 HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND SENDAI VIRUS NUCLEOPROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.750, 87.920, 45.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.300 - 1.700 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.21300 |
Structure solution method | CNS, done by rigid body refinement of starting model. |
Starting model (for MR) | pdb-entry 2vaa without peptide water and mutated side chains truncated to alanine. |
RMSD bond length | 0.006 |
RMSD bond angle | 25.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.300 | 1.720 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.045 | 0.456 |
Number of reflections | 61624 | 2029 * |
<I/σ(I)> | 34.4 | 3.5 |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 4.3 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 17 * | K/Na phosphate, MPD , pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | 2.1-2.2 (M) | ||
3 | 1 | reservoir | MPD | 2 (%) |