1FZJ
MHC CLASS I NATURAL MUTANT H-2KBM1 HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 134.910, 88.350, 45.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.900 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.22200 |
Structure solution method | CNS, done by rigid body refinement of starting model. |
Starting model (for MR) | pdb-entry 2vaa without peptide water and mutated side chains truncated to alanine. |
RMSD bond length | 0.009 |
RMSD bond angle | 25.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.056 | 0.405 |
Number of reflections | 43138 | 2102 * |
<I/σ(I)> | 20.3 | 2.5 |
Completeness [%] | 99.2 | 98.9 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 17 * | K/Na phosphate, MPD, pH 6.40, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | 2.1-2.2 (M) | ||
3 | 1 | reservoir | MPD | 2 (%) |