1FXU
PURINE NUCLEOSIDE PHOSPHORYLASE FROM CALF SPLEEN IN COMPLEX WITH N(7)-ACYCLOGUANOSINE INHIBITOR AND A PHOSPHATE ION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 4 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 94.110, 94.110, 94.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.180 - 2.200 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.24400 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.200 | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.097 * | |
| Total number of observations | 38538 * | |
| Number of reflections | 12786 | |
| Completeness [%] | 89.0 * | 87.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 * | 291 | 0.04M Tris/HCl buffer, PEG 4000, 11-16%, 0.08M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-12 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 10 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 4 | 1 | drop | reservoir solution | ||
| 5 | 1 | reservoir | PEG4000 | 11-16 (%(v/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 0.04 (M) | |
| 7 | 1 | reservoir | 0.08 (M) |
