1FW6
CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 1999-12-24 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.717, 113.496, 160.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.170 - 2.700 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.28000 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.200 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.082 | 0.324 |
Number of reflections | 49041 | |
<I/σ(I)> | 10.3 | |
Completeness [%] | 93.1 | 85.2 |
Redundancy | 2.4 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 20 * | Obmolova, G., (2000) Nature, 407, 703. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | cacodylate | ||
2 | 1 | 1 | (NH4)2SO4 | ||
3 | 1 | 1 | DTT | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 1 | PEG 4000 | ||
6 | 1 | 1 | ATP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.5-15 (mg/ml) | |
2 | 1 | reservoir | cacodylate | 100 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | PEG4000 | 18 (%) |