1FS0
COMPLEX OF GAMMA/EPSILON ATP SYNTHASE FROM E.COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795, 0.9793, 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 76.720, 176.090, 67.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.227 |
Rwork | 0.216 |
R-free | 0.26900 |
RMSD bond length | 0.085 |
RMSD bond angle | 1.650 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.053 | 0.256 |
Number of reflections | 26978 | |
<I/σ(I)> | 20 | |
Completeness [%] | 89.3 | 63.2 |
Redundancy | 6.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 22 * | 0.5M tartrate, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 22 * | 0.5M tartrate, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | tartrate | 0.5 (M) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) |