1FK7

STRUCTURAL BASIS OF NON-SPECIFIC LIPID BINDING IN MAIZE LIPID-TRANSFER PROTEIN COMPLEXES WITH RICINOLEIC ACID REVEALED BY HIGH-RESOLUTION X-RAY CRYSTALLOGRAPHY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1999-11-20
Detector	ENRAF-NONIUS
Spacegroup name	P 21 21 21
Unit cell lengths	24.810, 49.880, 69.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.205
Rwork	0.205
R-free	0.27400
Structure solution method	MR
Starting model (for MR)	(PDB CODE:1MZL)
RMSD bond length	0.014
RMSD bond angle	1.600
Data reduction software	PROFILE-FITTING (PROCEDURE)
Data scaling software	PROFILE-FITTING (PROCEDURE)
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall
High resolution limit [Å]	1.650
Rmerge	0.029
Total number of observations	21681 *
Number of reflections	8002
Completeness [%]	82.7
Redundancy	2.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		24.5-25.5 *	5.0M Na formate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	5.0 (M)