1FJM
Protein serine/threonine phosphatase-1 (alpha isoform, type 1) complexed with microcystin-LR toxin
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.740, 77.110, 130.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.100 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 2.100 * | |
| Rmerge | 0.056 * | 0.136 * |
| Total number of observations | 180676 * | |
| Number of reflections | 38768 * | |
| Completeness [%] | 99.5 * | 97.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 10 | 1 | reservoir | PEG4000 | 32 (%) | |
| 11 | 1 | reservoir | 1,7-diaminoheptane | 1.0 (M) | |
| 2 | 1 | drop | 0.5 (M) | ||
| 3 | 1 | drop | microcystin-LR | 4-fold molar excess | |
| 4 | 1 | drop | Tris-HCl | 50 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 6 | 1 | drop | 0.9 (mM) | ||
| 7 | 1 | drop | EGTA | 0.1 (mM) | |
| 8 | 1 | reservoir | Tris-HCl | 65 (mM) | |
| 9 | 1 | reservoir | 200 (mM) |
