1FIW
THREE-DIMENSIONAL STRUCTURE OF BETA-ACROSIN FROM RAM SPERMATOZOA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-01 |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 105.288, 105.288, 120.835 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.1996 |
Rwork | 0.197 |
R-free | 0.24200 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.242 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.068 | 0.298 |
Number of reflections | 20601 | |
<I/σ(I)> | 15.3 | |
Completeness [%] | 93.7 | 92.9 |
Redundancy | 4.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.2 * | 291 | sodium acetate, sodium formate, p-aminobenzamidine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | L-BAPNA | 0.375 (mM) | |
2 | 1 | drop | Tris-HCl | 0.1 (M) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | protein | 12mg | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | |
6 | 1 | reservoir | sodium formate | 2 (M) |