1FHV
CRYSTAL STRUCTURE ANALYSIS OF O-SUCCINYLBENZOATE SYNTHASE FROM E. COLI COMPLEXED WITH MG AND OSB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-07 |
Detector | APS-1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.800, 83.000, 57.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.770 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.22400 |
RMSD bond length | 0.014 |
RMSD bond angle | 16.030 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.045 | 0.112 |
Total number of observations | 319215 * | |
Number of reflections | 33959 * | |
<I/σ(I)> | 16.3 | |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 5.4 * | 296 | 5% Peg 4000, 3% Ethylene glycol,15 mM MgCl2, 1 mM OSB, 25 mM MES, microbatch, temperature 23K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 20 (mg/ml) | |
2 | 1 | 1 | PEG4000 | 5 (%) | |
3 | 1 | 1 | ethylene glycol | 3 (%) | |
4 | 1 | 1 | 15 (mM) | ||
5 | 1 | 1 | OSB | 1 (mM) | |
6 | 1 | 1 | MES | 25 (mM) |