1FFU
CARBON MONOXIDE DEHYDROGENASE FROM HYDROGENOPHAGA PSEUDOFLAVA WHICH LACKS THE MO-PYRANOPTERIN MOIETY OF THE MOLYBDENUM COFACTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.235, 193.884, 218.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.208 * |
| Rwork | 0.208 |
| R-free | 0.24500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.630 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.136 | 0.366 |
| Total number of observations | 570740 * | |
| Number of reflections | 138188 * | |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 91.1 | 76.6 |
| Redundancy | 4.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 4 * | potassium sodium tartrate, dihydrogen ammonium phosphate, methylpentanediol, dithioerythritol, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35.5 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 50 (mM) | |
| 3 | 1 | reservoir | sodium potassium tartrate | 1.1 (M) | |
| 4 | 1 | reservoir | ammonium phosphate | 0.3 (M) | |
| 5 | 1 | reservoir | MPD | 3 (%(w/v)) | |
| 6 | 1 | reservoir | DTE | 10 (mM) |
