1FFO
CRYSTAL STRUCTURE OF MURINE CLASS I H-2DB COMPLEXED WITH SYNTHETIC PEPTIDE GP33 (C9M/K1A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-27 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 |
Unit cell lengths | 47.219, 68.642, 81.531 |
Unit cell angles | 74.57, 73.08, 69.86 |
Refinement procedure
Resolution | 50.000 - 2.650 |
Rwork | 0.246 |
R-free | 0.29200 |
RMSD bond length | 0.008 |
RMSD bond angle | 25.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.125 | 0.206 * |
Total number of observations | 104494 * | |
Number of reflections | 24299 | |
<I/σ(I)> | 6.51 | |
Completeness [%] | 93.8 | 85.4 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 23% PEG8000, 6% DMSO, 0.8 M Glycine. 150 mM NaCl, 25mM MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MES | 25 (mM) | pH6.5 |
3 | 1 | reservoir | PEG8000 | 10-20 (%) | |
4 | 1 | reservoir | MES | 25 (mM) | pH6.5 |
5 | 1 | reservoir | dioxane | 1 (%) |