1FCH
CRYSTAL STRUCTURE OF THE PTS1 COMPLEXED TO THE TPR REGION OF HUMAN PEX5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-09 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.960, 73.510, 87.560 |
| Unit cell angles | 90.00, 120.78, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.200 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 18.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.227 |
| Number of reflections | 38298 | |
| <I/σ(I)> | 21.8 | |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | peptide solution was diluted 1:1 with reservoir * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.24 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | HEPES | 5 (mM) | |
| 4 | 1 | drop | peptide | 1.9 (mM) | |
| 5 | 1 | reservoir | Na citrate | 0.58 (M) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
