1F9U
CRYSTAL STRUCTURES OF MUTANTS REVEAL A SIGNALLING PATHWAY FOR ACTIVATION OF THE KINESIN MOTOR ATPASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-21 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.570, 78.040, 47.270 |
| Unit cell angles | 90.00, 105.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.217 * |
| Rwork | 0.217 |
| R-free | 0.26590 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.187 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 | |
| Total number of observations | 309408 * | |
| Number of reflections | 31822 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 93.5 | 89.5 |
| Redundancy | 9.72 | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | HEPES, PEG 2000 ME, Ethylen Glycol, magnesium chloride, Sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 ME | 20-26 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | HEPES | 50 (mM) |
