1F9K
WINGED BEAN ACIDIC LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-20 |
Detector | MARRESEARCH |
Spacegroup name | H 3 |
Unit cell lengths | 182.110, 182.110, 44.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WINGED BEAN BASIC LECTIN |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.110 | 0.442 * |
Total number of observations | 47085 * | |
Number of reflections | 11152 | |
Completeness [%] | 98.9 | 98.2 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.2 | 298 | Manoj, N., (1999) Acta Crystallogr., D55, 564. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 25 (mg/ml) | |
2 | 1 | 1 | sugar | in phosphate buffer(20-fold molar excess) | |
3 | 1 | 1 | PEG4000 | 30 (%(w/v)) |