1F9B
MELANIN PROTEIN INTERACTION: X-RAY STRUCTURE OF THE COMPLEX OF MARE LACTOFERRIN WITH MELANIN MONOMERS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-07 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.037, 99.815, 103.425 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.700 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.28700 |
RMSD bond length | 0.014 * |
RMSD bond angle | 2.000 * |
Data reduction software | DENZO |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 15.000 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.071 * | 0.142 * |
Total number of observations | 100340 * | |
Number of reflections | 18557 | |
<I/σ(I)> | 3.6 | |
Completeness [%] | 91.0 | 63 * |
Redundancy | 2.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 5 | 277 | 20mM Tris-Hcl, pH 5.0 concentration 50 mg/ml 10% ethanol soaked for 12hours in buffer containing DOPA, MICRODIALYSIS, temperature 4K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Tris-HCl | 20 (mM) | |
2 | 1 | 1 | protein | 50 (mg/ml) | |
3 | 1 | 2 | ethanol | 10 (%) |