1F90
FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 300 |
Detector technology | IMAGE PLATE |
Collection date | 1999-02-05 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.830, 72.760, 72.150 |
Unit cell angles | 90.00, 106.98, 90.00 |
Refinement procedure
Resolution | 99.000 - 2.600 |
R-factor | 0.18 * |
Rwork | 0.165 |
R-free | 0.26300 * |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.730 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.112 * | 0.583 |
Total number of observations | 19271 * | |
Number of reflections | 9377 * | |
<I/σ(I)> | 6.9 | |
Completeness [%] | 99.7 * | 97.15 |
Redundancy | 4.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | PEG-4000, 2-propanol, Na-citrate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-7 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 12-13 (%) | |
3 | 1 | reservoir | 2-propanol | 12-13 (%) | |
4 | 1 | reservoir | sodium citrate | 60 (mM) |