1F75
CRYSTAL STRUCTURE OF UNDECAPRENYL DIPHOSPHATE SYNTHASE FROM MICROCOCCUS LUTEUS B-P 26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-04 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.197, 60.161, 75.703 |
| Unit cell angles | 90.00, 105.69, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.190 |
| R-free | 0.24600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.032 | 0.168 |
| Total number of observations | 57323 * | |
| Number of reflections | 23530 * | |
| <I/σ(I)> | 24.3 | |
| Completeness [%] | 83.7 | 56.8 |
| Redundancy | 2.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 293 | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 7 | 1 | reservoir | lithium sulfate | 1.0 (M) |
