1F6L
VARIABLE LIGHT CHAIN DIMER OF ANTI-FERRITIN ANTIBODY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-19 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 52.214, 52.214, 157.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 500.000 - 2.800 |
Rwork | 0.191 |
R-free | 0.25700 |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.340 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.099 | 0.323 |
Total number of observations | 40650 * | |
Number of reflections | 3589 * | |
Completeness [%] | 96.3 | 90.5 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 4 * | PEG 400, ammonium sulfate, hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | domain | 9-10 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 4 (%) | |
3 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.2 |