1F2O
CRYSTAL STRUCTURE OF THE STREPTOMYCES GRISEUS AMINOPEPTIDASE COMPLEXED WITH L-LEUCINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.990, 61.990, 146.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.147 |
| Rwork | 0.147 |
| R-free | 0.17500 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.083 | 0.176 |
| Number of reflections | 31106 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 95.8 | 84.2 * |
| Redundancy | 5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | used seeding * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 6 (mM) | ||
| 5 | 1 | drop | L-Leucine | 200 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 24 (%(w/v)) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.1 (M) |
