1F1U
CRYSTAL STRUCTURE OF HOMOPROTOCATECHUATE 2,3-DIOXYGENASE FROM ARTHROBACTER GLOBIFORMIS (NATIVE, LOW TEMPERATURE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 1998-08-29 |
Detector | CUSTOM-MADE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.800, 59.040, 102.250 |
Unit cell angles | 90.00, 119.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.162 |
Rwork | 0.162 |
R-free | 0.18500 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.630 |
High resolution limit [Å] | 1.500 | 1.510 |
Rmerge | 0.081 * | 0.271 |
Number of reflections | 111428 | |
<I/σ(I)> | 22 | |
Completeness [%] | 96.9 | 93.4 * |
Redundancy | 3.0 * | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.5 * | 18 * | Peg 8000, Mg Acetate Na cacodylate. Data was collected with added glycerol as a cryoprotectant., pH 6.8, Batch crystallization, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 100 (mg/ml) | |
2 | 1 | 2 | PEG8000 | 6-10 (%) | |
3 | 1 | 2 | magnesium acetate | 0.2 (M) | |
4 | 1 | 2 | sodium cacodylate | 100 (mM) | pH6.5 |