1EWM

THE CYSTEINE PROTEASE CRUZAIN BOUND TO WRR-112

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-05-18
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	43.258, 51.700, 45.271
Unit cell angles	90.00, 115.20, 90.00

Refinement procedure

Resolution	25.000 - 2.000
R-factor	0.173
Rwork	0.173
R-free	0.20600
RMSD bond length	0.012
RMSD bond angle	2.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.094	0.167
Number of reflections	11831
<I/σ(I)>	6
Completeness [%]	95.8	91.3
Redundancy	2.75	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.9M NaCitrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K