Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 22 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.154, 79.114, 99.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.184 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.082 |
Total number of observations | 133137 * |
Number of reflections | 23549 |
<I/σ(I)> | 10 |
Completeness [%] | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 24 (%) | |
2 | 1 | reservoir | 0.2 (M) | ||
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
4 | 1 | drop | protein | 0.400 (mM) | |
5 | 1 | drop | androstenedione | 0.800 (mM) | or 9-aminophenanthrene 0.400mM |