1EUJ
A NOVEL ANTI-TUMOR CYTOKINE CONTAINS A RNA-BINDING MOTIF PRESENT IN AMINOACYL-TRNA SYNTHETASES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-13 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.010, 38.340, 80.990 |
| Unit cell angles | 90.00, 112.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.208 * |
| Rwork | 0.208 |
| R-free | 0.23200 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.580 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.282 |
| Total number of observations | 96335 * | |
| Number of reflections | 31502 | |
| <I/σ(I)> | 31.3 | |
| Completeness [%] | 88.2 | 37.9 |
| Redundancy | 3.1 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 294 | 20% PEG 4000, 100 mM NaAcetate, 15 mM MgCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 21K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 0.5 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 7 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 8 | 1 | reservoir | 15 (mM) |
