1EUE

RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-01-15
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	46.230, 70.770, 72.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.930 - 1.800
R-factor	0.197
Rwork	0.197
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1awp
RMSD bond length	0.010
RMSD bond angle	21.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.200	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.065	0.480
Number of reflections	21091
<I/σ(I)>	20.6	1.8
Completeness [%]	93.0	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	277	drop solution is mixed 1:1 by volume with precipitant solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG8000	20 (%(w/v))	precipitant
3	1	reservoir	magnesium acetate	0.2 (M)	precipitant
4	1	reservoir	PIPES	0.1 (M)	precipitant