1ESZ
STRUCTURE OF THE PERIPLASMIC FERRIC SIDEROPHORE BINDING PROTEIN FHUD COMPLEXED WITH COPROGEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-11 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 63 |
| Unit cell lengths | 86.090, 86.090, 91.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| R-free | 0.24100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 | 0.102 |
| Number of reflections | 25292 | |
| <I/σ(I)> | 35.1 | |
| Completeness [%] | 95.6 | 90.1 * |
| Redundancy | 90.1 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | Na/K phosphate, HEPES, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.3 (mg/ml) | |
| 2 | 1 | drop | disodium/potassium phosphate | 0.8 (M) | |
| 3 | 1 | drop | HEPES | 0.05 (M) | pH7.5 |
| 4 | 1 | reservoir | disodium/potassium phosphate | 1.6 (M) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
