1EKF
CRYSTALLOGRAPHIC STRUCTURE OF HUMAN BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE (MITOCHONDRIAL) COMPLEXED WITH PYRIDOXAL-5'-PHOSPHATE AT 1.95 ANGSTROMS (ORTHORHOMBIC FORM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 223 |
Detector technology | CCD |
Collection date | 1999-04-20 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.385, 105.032, 107.016 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.290 - 1.950 |
R-factor | 0.232 |
Rwork | 0.222 |
R-free | 0.26000 |
Starting model (for MR) | E. COLI BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE. |
RMSD bond length | 0.009 |
RMSD bond angle | 1.584 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.290 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.065 | 0.414 |
Number of reflections | 48329 | |
<I/σ(I)> | 21.34 | |
Completeness [%] | 83.8 | 63.8 |
Redundancy | 4.81 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG1500, HEPES, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5 (mg/ml) | |
2 | 1 | drop | HEPES | 50 (mM) | |
3 | 1 | drop | dithiothreitol | 20 (mM) | |
4 | 1 | drop | EDTA | 50 (mM) | |
5 | 1 | reservoir | PEG1500 | 22-30 (%) | |
6 | 1 | reservoir | HEPES | 100 (mM) | |
7 | 1 | reservoir | dithiothreitol | 20 (mM) |