1EBH

OCTAHEDRAL COORDINATION AT THE HIGH AFFINITY METAL SITE IN ENOLASE; CRYSTALLOGRAPHIC ANALYSIS OF THE MG++-ENZYME FROM YEAST AT 1.9 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	72.500, 73.200, 89.100
Unit cell angles	90.00, 104.40, 90.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.19
RMSD bond length	0.019 *
RMSD bond angle	2.200
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.041 *	0.290 *
Total number of observations	77897 *
Number of reflections	53410 *	6194 *
Completeness [%]	75.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	16 (%(w/w))
2	1	1		0.25 (M)
3	1	1	HEPPS	0.04 (M)
4	1	1		0.5 (mM)