1E15
Chitinase B from Serratia Marcescens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.135, 106.586, 187.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.870 - 1.900 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.21200 |
Structure solution method | MIRAS |
RMSD bond length | 0.016 |
RMSD bond angle | 24.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.087 | 0.344 |
Number of reflections | 88458 | |
<I/σ(I)> | 11.5 | 3.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 | 0.25 M LI2SO4, 1.0 M(NH4)2SO4, CITRATE BUFFER PH 5.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | citrate | 50 (mM) | |
3 | 1 | reservoir | 0.5 (M) | ||
4 | 1 | reservoir | ammonium sulfate | 0.25 (M) |