1DXL
Dihydrolipoamide dehydrogenase of glycine decarboxylase from Pisum Sativum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.560, 108.330, 202.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.150 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.32500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lad |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 23.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS (0.4) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.063 * | 0.254 * |
| Number of reflections | 33527 | |
| <I/σ(I)> | 8.6 | 3 |
| Completeness [%] | 86.7 * | 77.3 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 20 * | 15 % PEG MME 5K, 100 MM MES PH 5, 0.45 % HEPTYL-B-D-THIOGLUCOPYRANOSIDE, 2MM POTASSIUM LIPOATE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | reservoir | mPEG5000 | 12 (%(w/v)) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | |
| 4 | 1 | reservoir | heptyl-beta-D-thioglucopyranoside | 0.45 (%) |
