1DW9
Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-15 |
| Detector | ARGONNE APS-1 |
| Wavelength(s) | 0.9465, 0.9793, 0.9795, 1.033, 1.078, 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.340, 81.030, 82.300 |
| Unit cell angles | 70.30, 72.20, 66.40 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.15 * |
| Rwork | 0.150 |
| R-free | 0.18900 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.039 * | 0.198 * |
| Total number of observations | 524489 * | |
| Number of reflections | 187107 | |
| <I/σ(I)> | 26.4 | 3.9 |
| Completeness [%] | 94.2 | 80.6 |
| Redundancy | 2.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 18 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 2.1 (M) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) |
