1DVU
CRYSTAL STRUCTURE OF HUMAN TRANSTHYRETIN IN COMPLEX WITH DIBENZOFURAN-4,6-DICARBOXYLIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-10 |
| Detector | MACSCIENCE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.140, 86.000, 65.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.22000 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.278 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.700 | 1.600 |
| Rmerge | 0.075 | 0.316 |
| Total number of observations | 157123 * | |
| Number of reflections | 26953 | |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 98.4 | 89.2 |
| Redundancy | 5.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | potassium chloride, potassium phosphate, ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | phosphate | 100 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 1 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) |
