1DVN
LATENT FORM OF PLASMINOGEN ACTIVATOR INHIBITOR-1 (PAI-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-06 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.581, 46.697, 61.966 |
| Unit cell angles | 90.00, 107.11, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.204 * |
| Rwork | 0.236 |
| R-free | 0.26900 * |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (98.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.124 | 0.340 |
| Total number of observations | 292887 * | |
| Number of reflections | 24999 * | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 96.0 | 92.2 |
| Redundancy | 4.7 * | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 * | 293 | PEG1000, MPD, MgSO4, Tris, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | PEG1000 | 2-8 (%) | |
| 5 | 1 | reservoir | Tris | 20 (mM) | |
| 6 | 1 | reservoir | MPD | 1.5 (%) |
