1DT1
THERMUS THERMOPHILUS CYTOCHROME C552 SYNTHESIZED BY ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.900, 86.900, 31.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| R-factor | 0.21 |
| Rwork | 0.182 |
| R-free | 0.26200 |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 0.905 * |
| Data reduction software | XFIT |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.304 |
| Total number of observations | 92080 * | |
| Number of reflections | 15100 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 297 | 36% MPEG 5K (w/v), 0.2M Im-mat 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 24K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | mPEG5000 | 36 (%(w/w)) | |
| 5 | 1 | reservoir | imidazole-malate | 0.2 (M) |
