1DQ1
Calcium;Calcium concanavalin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-20 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 62.964, 87.312, 88.936 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.150 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.27600 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.250 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.104 | 0.375 |
Number of reflections | 13518 | |
<I/σ(I)> | 14.58 | 5.2 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 2.1 M ammonium sulphate, 120 mM sodium acetate, 100 mM calcium, prepared by soaking of locked, metal-free concanavalin A crystals (1DQ0) with calcium, pH 7.0, temperature 293.0K |