1DOB
THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS
Experimental procedure
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.900, 145.940, 88.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
R-factor | 0.205 |
Rwork | 0.205 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.840 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.119 * |
Number of reflections | 27601 * |
Completeness [%] | 87.0 * |
Redundancy | 3.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | free interface diffusion * | 7.5 * | 4-20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | drop | p-hydroxybenzoate | 2 (mM) | |
3 | 1 | drop | FAD | 0.04 (mM) | |
4 | 1 | drop | EDTA | 0.3 (mM) | |
5 | 1 | drop | sodium sulphite | 30 (mM) | |
6 | 1 | drop | phosphate | 0.1 (M) | |
7 | 1 | reservoir | ammonium sulfate | 70 (%sat) | |
8 | 1 | reservoir | phosphate | 0.1 (M) |