1DL5
PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-06-11 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.060, 98.910, 76.860 |
Unit cell angles | 90.00, 105.66, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.800 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.20300 |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.380 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.850 |
High resolution limit [Å] | 1.650 * | 1.800 |
Rmerge | 0.053 | 0.237 |
Number of reflections | 66308 | |
<I/σ(I)> | 18.3 | 10 |
Completeness [%] | 97.6 | 95.7 |
Redundancy | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 18 * | THREE MICROLITERS OF PROTEIN AT A CONCENTRATION OF 6.3MGS/ML IN 10MM TRIS PH 8.0, 200MM NACL, 5% GLYCEROL, LMM DTT, 5MM BME AND 0.1MM EDTA WERE MIXED WITH THREE MICROLITERS OF A RESERVOIR SOLUTION OF 28% PEG400, 100MM SODIUM ACETATE PH 4.5, AND 100MM CADMIUM CHLORIDE AND EQUILIBRATED AGAINST THE SAME., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG400 | 28 (%(v/v)) | |
2 | 1 | reservoir | 100 (mM) | ||
3 | 1 | reservoir | 100 (mM ) | or ZnSO4 |