1DJS
LIGAND-BINDING PORTION OF FIBROBLAST GROWTH FACTOR RECEPTOR 2 IN COMPLEX WITH FGF1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-06-17 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9919, 0.9793, 0.9791, 0.9641 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 129.970, 129.970, 129.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.400 |
| R-factor | 0.227 |
| Rwork | 0.221 |
| R-free | 0.31500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MADSYS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.402 |
| Total number of observations | 180297 * | |
| Number of reflections | 41549 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | reservoir solution | same volume of 2:1 diluted reservoir solution | |
| 3 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 10 (mM) |
