1DGE

AN ALKALI METAL ION SIZE-DEPENDENT SWITCH IN THE ACTIVE SITE STRUCTURE OF DIALKYLGLYCINE DECARBOXYLASE

Experimental procedure

Spacegroup name	P 64 2 2
Unit cell lengths	153.500, 153.500, 86.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.195
RMSD bond length	0.013
RMSD bond angle	2.400
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.108 *
Total number of observations	62340 *
Number of reflections	14856 *
Completeness [%]	97.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		referred to J.Mol.Biol. 222.873-875 1991 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-60 (mg/ml)
2	1	drop	potassium phosphate	5 (mM)
3	1	drop	PLP	0.015 (mM)
4	1	drop	sodium azide	0.02 (%(w/v))
5	1	reservoir	PEG4000	15 (%(w/v))
6	1	reservoir	morpholinoethanesulfonic acid	15 (mM)
7	1	reservoir	potassium hydroxide		to neutralize
8	1	reservoir	sodium pyruvate	75-150 (mM)
9	1	reservoir	PLP	0.015 (mM)