1DFJ
RIBONUCLEASE INHIBITOR COMPLEXED WITH RIBONUCLEASE A
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994 |
Detector | RIGAKU RAXIS II |
Spacegroup name | I 4 |
Unit cell lengths | 133.300, 133.300, 86.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.500 |
R-factor | 0.194 |
Rwork | 0.194 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.826 |
Data reduction software | DENZO |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 * |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.100 |
Total number of observations | 111046 * |
Number of reflections | 18859 |
Completeness [%] | 71.4 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7 * | 21 * | Kobe, B., (1994) J.Mol.Biol., 241, 288. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium salfate | 1.3 (M) | |
2 | 1 | reservoir | sodium acetate | 100 (mM) | |
3 | 1 | reservoir | DTT | 20 (mM) | |
4 | 1 | drop | RI | 22 (mg/ml) | |
5 | 1 | drop | potassium phosphate | 16 (mM) | |
6 | 1 | drop | DTT | 16 (mM) | |
7 | 1 | drop | RNase A | 5.6 (mg/ml) |